NGS introduce

NGS and Microarray

Microarray可偵測的訊號範圍受限於雜訊、探針飽和，NGS可透過調整reads number至研究者實驗所需深度，NGS的訊號偵測範圍幾乎是unlimited dynamic range。

PE and SE

Advantage of PE

• More accurate
  • detect indel
  • higher SNV calls
• Removal of PCR duplication